Karl Stefanovic appeared to make a cheeky on-air gag about his former co-host Lisa Wilkinson during Tuesday's Today Show.
The TV host joked about how he was feeling 'bullied in the workplace' during a discussion with co-host Allison Langdon and entertainment reporter Brooke Boney.
The 47-year-old's comments come as Lisa is set to release her tell-all book on Wednesday, which features her sacking from Nine and falling out with Karl.
Cheeky: Karl Stefanovic amused himself and his Today co-hosts as he carefully quipped that he 'feels bullied in the workplace' - the day before Lisa Wilkinson's tell-all memoir lands on bookshelves
In the incident this morning, Boney made a comment about Allison and Karl, who were making quips about one another, and said: 'I hate it when the mother and father fight. It's really uncomfortable.'
'Tough audience,' replied Karl as he fixed up his tie in a nervous gesture.
Allison proceeded to ask her colleague if he was okay, to which he responded lightheartedly: 'I feel like I'm being bullied in the workplace.'
Playful banter: The TV host joked about how he was feeling 'bullied' during a discussion with co-host Allison Langdon and entertainment reporter Brooke Boney, who were both making lighthearted quips about him
The demeanour of Karl's two co-workers quickly changed, with Allison telling him 'not to use that word'.
Brooke began giggling during the exchange before she asked Karl to 'wake up' to himself and 'pull' his head in.
The comments between the trio were made just hours before Lisa controversial memoir - It Wasn't Meant To Be Like This - will be released.
In the book, Lisa talks about being sacked by Channel Nine and falling out with Karl over a pay dispute between the pair.
Secrets unveiled: The comments between the trio were made as Lisa Wilkinson's controversial memoir - It Wasn't Meant To Be Like This - is to be released in bookstores tomorrow
She alleges Karl proposed in August 2015 they join forces to renegotiate their Nine contracts.
But she claims he ended up ditching this idea and instead played rival networks