Her co host Kyle Sandilands has been known to gush over her 'amazing boobs'.
And on Wednesday KIIS FM host Jackie O Henderson revealed the actual size of her breasts.
'They went from a B to a C', said the busty mother-of-one referring to her breasts post children.
What would you guess? Jackie O Henderson reveals the size of her breasts live on air... and it's not what you think
'More like a DD!' Laughed Karl.
Jackie insisted they were not but Karl kept on pushing her breasts they were larger than she claimed.
This is the second time during the week, Karl has talked about Jackie's anatomy.
'They went from a B to a C'!' said the busty mother-of-one referring to her breasts post children. Pictured with daughter Kitty
On Tuesday he was at it again as he enthused about her 'cans' while he critiqued her Instagram account.
'Boobs are amazing!' Kyle enthused as he eyeballed his 42-year old's selfie.
The image, which was posted to Jackie's account back in October, shows the mother-of-one flaunting extreme cleavage in a plunging bikini as she sits beside her pool.
'They're big boobs,' mused Karl.
'Did you pull your top down?' he questioned.
'No. I didn't,' insisted Jackie seemingly upset.
'Boobs are amazing!' Kyle enthused as he