England's No 1 Karen Bardsley is out of the rest of the Women's European Championship after scans revealed she has broken her leg.
Liverpool goalkeeper Siobhan Chamberlain will step up to play in Thursday's semi-final against hosts Holland after Bardsley suffered the injury in the historic quarter-final win over France.
Bardsley, 32, refused a stretcher in Deventer but later limped off in agony before tests results on Tuesday showed a fractured fibula.
Karen Bardsley has been ruled out of the rest of the women's European Championship
The Manchester City stopper has not conceded a goal at this Euro and her absence is a major blow to manager Mark Sampson's hopes of a first major tournament triumph.
Chamberlain is an able deputy, though, and played the full 90 minutes against Portugal in the group stage last week before a 14-minute outing in the France win.
Bardsley will remain with the squad in the build-up to Thursday night's semi-final in Enschede but England cannot call up a replacement.
Veteran defender Casey Stoney, who has been persistently troubled by injury in Holland, was the only other England player not to train at their Utrecht base on Tuesday.