Conservative Party chairman James Cleverly has immediately rejected a fresh appeal from Nigel Farage to form a general election Leave Alliance.
The Tories and the Brexit Party are now on a collision course for a showdown at the ballot box on December 12 after Mr Cleverly and other senior Tories dismissed Mr Farage's offer of a pact.
Mr Farage said he was ready to fight against the Tories in every seat across England, Scotland and Wales if Boris Johnson refused to ditch his Brexit deal and back a 'clean break' from Brussels.
He gave Mr Johnson a deadline of November 14 for the two parties to come to some sort of non-aggression arrangement.
But Mr Cleverly turned down the offer as he made clear that the Tories do not so pacts with other parties.
He said: 'A vote for Farage risks letting Jeremy Corbyn into Downing Street via the back door - and the country spending 2020 having two referendums on Brexit and Scottish independence.
'It will not get Brexit done - and it will create another gridlocked Parliament that doesn't work.'
Meanwhile, a senior Tory source was unequivocal as they said: 'A vote for anyone other than Boris risks Brexit. We don't do electoral pacts.'
Nigel Farage, pictured in London today, said the Brexit Party would stand 500 candidates across the country unless Boris Johnson dropped his divorce deal and agreed to a pact
But James Cleverly, the Tory Party chairman pictured in Downing Street on October 29, immediately rejected Mr Farage's terms
Mr Farage had set out his demands at the Brexit Party's official election campaign launch in London this morning.
He said that ff the government does not agree to his terms 'then the Brexit Party will be the only party standing in these elections that actually represents Brexit'.
He warned: 'We will contest every seat in England, Scotland and Wales. Don't doubt that we are ready.'
He added: 'I will say this to Boris Johnson: drop the deal, drop the deal because it is not Brexit, drop the deal because as weeks go by and people discover what it is you will have signed up, they will not like it.'
Robert Jenrick, the Communities Secretary, said before Mr Farage reiterated his offer that the Tories are 'not interested in doing any pacts with the Brexit Party or indeed with anybody else'.
The Conservatives last night pledged millions of pounds to run-down towns to help them revitalise their high streets and boost business.
Communities Secretary Robert Jenrick announced £16.4million to be shared out between 100 towns from Dewsbury to Penzance.