Insulate Britain today urged drivers not to use the M25 as they threatened to bring chaos to the motorway once again tomorrow with a series of road blockades.
The environmental campaigners also asked police to refuse to arrest them and warned their 'non-violent civil resistance' will restart on the road from 7am tomorrow.
Insulate Britain added that if motorists do still choose to use the M25 tomorrow then their speed should be 'reduced to 20 mph to minimise the risk of accidents'.
It comes after the London-based activists were effectively banned from all major roads in England yesterday following a landmark High Court ruling.
The protesters have brought chaos to motorways and A roads over the last six weeks with hundreds of arrests made – but the Metropolitan Police has still charged no one.
In their latest demonstration, the eco-zealots brought parts of London to a standstill once again yesterday - several of them glueing their hands, feet or faces to roads.
Insulate Britain activists once again caused chaos in London yesterday as they brought traffic traffic to a standstill on Bishopsgate near Liverpool Street station
An Insulate Britain spokesman said today: 'In light of a national injunction covering England's highways, Insulate Britain has declared the M25 a site of nonviolent civil resistance and called for motorway traffic to be slowed.
'You can't imprison a flood, there are no unlimited fines against a famine, you can't bankrupt a fire. You can imprison the ordinary people of Britain, yet the lives of our children and those of all future generations hang in the balance.
'By refusing to insulate Britain's homes, our government is also condemning thousands to death through fuel poverty this winter, while countless families will once again be cold and hungry.
'We are not concerned with endless injunctions. We are not concerned with our fears. We are concerned with fulfilling our duties and responsibilities at this 'period of consequence'.'
The group added that the M25 will 'become a place of nonviolent civil resistance to stop our government committing crimes against humanity' from 7am tomorrow.
The Insulate Britain spokesman continued: 'This government, our government, is actively pursuing policies that will lead to the destruction of our country due to climate catastrophe.
'In a free society, citizens have the right and a duty to rebel against plans which will knowingly result in the deaths of millions.
'Insulate Britain acknowledges the inconvenience and irritation we are causing to the public in our campaign, we ask that you understand that the days of disruption are necessary to force a government to fulfil its most basic of duties to protect and defend its people.
'Insulate Britain considers the UK Government to be in treasonous betrayal of this country.'
The group has set out three demands – the first being that people 'do not use the M25, or if they do, speeds are reduced to 20 mph to minimise the risk of accidents'.
Protesters from Insulate Britain block a road near Canary Wharf in East London yesterday
Climate activists from Insulate Britain are pulled off the road near Southwark Bridge yesterday
The second is that National Highways 'acts on its responsibilities to keep the public safe by enforcing this speed limit'.