Ms Brilhante was handed a declaration of "persona non grata" by the Venezuelan Minister of Foreign Affairs Jorge Arreaza on February 24. The EU ambassador was given 72 hours to leave the country in response to a new round of EU sanctions.
This comes after the EU foreign ministers agreed to sanction 19 Venezuelan officials, bringing the overall total to 55.
Mr Arreaza said: "Today, by decision of President Nicolas Maduro, we have personally delivered to Mrs Isabel Brilhante a declaration of persona non grata.
"She was given 72 hours to leave Venezuelan territory."
The Minister added the reason was due to the "55 sanctions" imposed on the country by the EU.Insurance Loans Mortgage Attorney Credit Lawyer
Venezuelan president Nicolas Maduro (Image: Getty)
EU ambassador Isabel Brilhante (Image: PA)
He said: "We do it because there are 55 sanctions, as if they had moral authority, which they don't have and they are not legitimate to impose any kind of punishment.
"Venezuela is irrevocably free and independent and bases fit moral heritage on its values of freedom, equality, justice and international peace in the doctrine of Simon Bolivar."
Bolivar is known as "the Liberator" who helped gain independence for Latin American countries.
Venezuela has become a rogue state under President Maduro, with political and economic chaos leading to mass protests and emigration.
READ MORE: EU poised to 'legitimise despotic regime in dreadful error’
Venezuelan Minister of Foreign Affairs Jorge Arreaza (Image: Getty)
Around 5 million people are estimated to have left Venezuela in recent years while the UN estimates 9,000 people have been subjected to extrajudicial killing.
Two members of the National Assembly, the governor of Zulia